Japanese media website, Goo, asked their manga reader users: Which characters deserve to be addressed as -sama? With a total of 500 responses and 250 of them being males and 250 being females, here are the results:
15. Chikage Kazama (Hakuoki)
14. Reinhard von Lohengramm (Legend of the Galactic Heroes)
13. Hozuki (Hoozuki no Reitetsu)
12. Vearn (Dragon Quest: Dai no Daiboken)
11. Tsunade (Naruto)
10. Kaito Kuroba (Detective Conan)
9. Heart (Hokuto no Ken)
8. Haku (Spirited Away)
7. Haman Karn (Mobile Suit Zeta Gundam)
6. Oscar François de Jarjayes (Rose of Versailles)
5. Dio Brando (Jojo’s Bizarre Adventure)
4. Keigo Atobe (Prince of Tennis)
3. Yupa (Nausicaä of the Valley of the Wind)
2. Doronjo (Yatterman)
1. Frieza (Dragon Ball Z)